Identification of roxarsonemetabolites produced in the system: Soil–chlorinated water–light by using HPLC-ICP-MS/ESI-MS, HPLC-ESI-MS/MS and High Resolution Mass Spectrometry (ESI-TOF-MS)

Identification of chemical constituents in cuscuta chinensis using HPLC-ESI/Q-TOF MS/MS

AIM: To identify the chemical constituents in Cuscuta Chinensis. METHODS: A High-performance liquid chromatography (HPLC) method combined with quadrupole-time-of-flight-mass spectrometry (QTOF-MS) has been developed for precise separation and structural identification of components in Cuscuta Chinensis. Mass spectrometry was performed in reflective time-of-flight using electron spraying ionizat...

Identification of mammalian cell lines using MALDI-TOF and LC-ESI-MS/MS mass spectrometry.

Direct mass spectrometric analysis of complex biological samples is becoming an increasingly useful technique in the field of proteomics. Matrix-assisted laser desorption/ionization mass spectroscopy (MALDI-MS) is a rapid and sensitive analytical tool well suited for obtaining molecular weights of peptides and proteins from complex samples. Here, a fast and simple approach to cellular protein p...

Analysis of Pharmaceuticals and Biomolecules Using Hplc Coupled to Icp-ms and Esi-ms

Analysis of pharmaceuticals and biomolecules using HPLC coupled to ICPMS and ESI-MS Andrew James Cartwright The work described within this thesis explores the use of HPLC coupled with ICPMS and ESI-MS in order to develop novel methods which overcome specific analytical challenges in the pharmaceutical industry. A membrane desolvation interface has been evaluated for coupling high performance li...

HPLC-ESI-MS/MS of imidazole alkaloids in Pilocarpus microphyllus.

Pilocarpine, an important imidazole alkaloid, is extracted from the leaves of Pilocarpus microphyllus (Rutaceae), known in Brazil as jaborandi and used mainly for the treatment of glaucoma. Jaborandi leaves also contain other imidazole alkaloids, whose pharmacological and physiological properties are unknown, and whose biosynthetic pathways are under investigation. In the present study, a HPLC ...

A Highly Accurate Mass Profiling Approach to Protein Biomarker Discovery Using HPLC-Chip/ MS-Enabled ESI-TOF MS